Single Cell Metabolism
SCENITH™ is a method to functionally profile metabolism in multiple cells in parallel by flow cytometry.
We developed protocols for different type of samples, a full set of multiple metabolic inhibitors and an adapted monoclonal antibody.
The SCENITH™ kit enables functional analysis of cellular metabolism at single-cell resolution using flow cytometry. This innovative methodology, co-invented by Dr. Rafael Argüello, measures protein synthesis as an indicator of cellular metabolic activity. The kit allows analysis of all cell types, in any animal model organism (e.g. Fly, Fish, Mouse, Human) including rare cells, in whole blood samples, rapidly ex-vivo avoiding metabolic biases introduced by cell culture media pmc.ncbi.nlm.nhttps
Kit Components:
· Monoclonal anti-puromycin antibody (clone R4743L-E8) conjugated to multiple fluorophores, ready to use.
· Panels of metabolic inhibitors tailored to assess metabolic activity and metabolic dependencies (e.g. glucose and mitochondrial dependency).
· Optimized protocols for use with standard flow cytometers.
· The kit contains sufficient reagents for 250 individual stainings (experimental conditions). Since each biological sample requires 5 SCENITH™ conditions, this corresponds to approximately 50 biological samples.
The fluorescently conjugated anti puromycin antibody is exclusively commercialized as part of the patented SCENITH™ technology and is not available for individual sale outside of the validated kit.
Some Applications:
⊕ Functional Metabolic profiling of immune cells in peripheral blood.
⊕ Analysis of metabolic plasticity in tumor cells and co-cultures.
⊕ Study of metabolic heterogeneity in rare cell populations.
Technical Specifications:
⊕ The functional analysis assay that requires the minimal number of cells.
⊕ Only one fluorescent channel occupied by the readout, and antibody available in fluorochromes compatible with all standard flow cytometers.
⊕ Reproducible results in a short timeframe.
⊕ Measures cellular metabolic activity at single-cell resolution using protein synthesis as a readout.
⊕ Assesses both glucose and mitochondrial dependency in any cell type, including rare cells.
⊕ No need for cell isolation, working directly in heterogeneous samples, in whole blood or rapidly ex-vivo in cell culture media to preserve physiological conditions.
⊕ Panels of metabolic inhibitors are pre-optimized, flexible, and customizable for different experimental needs.
⊕ Each lot is rigorously tested and validated to ensure reproducible, reliable results and minimize experimental failure.
